Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus
Mamuna Hafeez,
Syeda Mona Hassan,
Shahzad Sharif Mughal,
Muneeza Munir,
Muhammad Kamran Khan
Issue:
Volume 5, Issue 4, December 2020
Pages:
69-79
Received:
22 June 2020
Accepted:
29 October 2020
Published:
19 November 2020
Abstract: Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. The antifungal and antioxidant components of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW)in 80% methanolic solvent system. Phytochemical analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of A. esculentus leaves and seeds was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (29 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (52.5%). Overall results of the present study showed that 80% methanolic leaves extracts of A. esculentus possesses very good antioxidant, antimicrobial and cytotoxic properties.
Abstract: Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. Th...
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Breeding Objective, Selection Criteria and Breeding Practice of Indigenous Goat Types in Ancher and Gemechis Districts, Eastern Ethiopia
Mohammed Yousuf,
Tesfaye Getachew,
Elias Bayou
Issue:
Volume 5, Issue 4, December 2020
Pages:
80-89
Received:
18 November 2020
Accepted:
1 December 2020
Published:
11 December 2020
Abstract: This study was aimed to generate basic information on breeding objective, selection criteria and breeding practice of indigenous goat type under farmer’s management condition in Ancher and Gemechis districts, Eastern Ethiopia. The study was performed based on household survey. About 240 households (120 from each district) were used for household survey. Appearance, color, testicular characteristics and horn were most important traits considered by farmers to choose breeding bucks in Ancher and Gemechis district. Appearance and coat color ranked as first and second, respectively followed by better milk yield in Ancher and twinning ability in Gemechis district as third criteria for selection breeding does. The average age at first sexual maturity of male in Ancher and Gemechis district were 7.6±0.07 and 7.4±0.06 months, respectively, in the meantime female goats were the average age at first sexual maturity in Ancher and Gemechis districts was 7.8±0.07 and 7.6±0.06 months, respectively. The mean of age at first kidding for Ancher and Gemechis districts goat 12.9±0.15 and 12.5±0.14 months, respectively. Mean estimate of effective population size and mean rate of inbreeding was 2.4 and 0.21, respectively when a household flock is herded alone and under random mating. Therefore, any breed improvement strategies that are intended to be implemented in the study area and otherwise- where should consider the traditional breeding practices and breeding objectives of the community.
Abstract: This study was aimed to generate basic information on breeding objective, selection criteria and breeding practice of indigenous goat type under farmer’s management condition in Ancher and Gemechis districts, Eastern Ethiopia. The study was performed based on household survey. About 240 households (120 from each district) were used for household su...
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