Volume 5, Issue 4, December 2020, Page: 69-79
Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus
Mamuna Hafeez, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Syeda Mona Hassan, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Shahzad Sharif Mughal, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Muneeza Munir, Department of Chemistry, Lahore Garrison University, Lahore, Pakistan
Muhammad Kamran Khan, Department of Botany, Government College University, Faisalabad, Pakistan
Received: Jun. 22, 2020;       Accepted: Oct. 29, 2020;       Published: Nov. 19, 2020
DOI: 10.11648/j.cbe.20200504.11      View  106      Downloads  22
Abstract
Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. The antifungal and antioxidant components of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW)in 80% methanolic solvent system. Phytochemical analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of A. esculentus leaves and seeds was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (29 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (52.5%). Overall results of the present study showed that 80% methanolic leaves extracts of A. esculentus possesses very good antioxidant, antimicrobial and cytotoxic properties.
Keywords
Antioxidant, Antibacterial, Scavenging, Cytotoxicity, Potential
To cite this article
Mamuna Hafeez, Syeda Mona Hassan, Shahzad Sharif Mughal, Muneeza Munir, Muhammad Kamran Khan, Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus, Chemical and Biomolecular Engineering. Vol. 5, No. 4, 2020, pp. 69-79. doi: 10.11648/j.cbe.20200504.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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