The importance of biodegradation in the context of dye removal is presented as a function of the need to use enzyme immobilization as a key technology for effluent treatment. Immobilization is based on the need to improve the stability and recyclability of the biocatalyst in relation to the free enzyme, in addition to a lower risk of product contamination as an advantage. The present study aims to evaluate the biodegradation of textile dyes using radish peroxidase immobilized by physical adsorption method on coconut fiber, as well as its characterization in relation to pH, temperature, and infrared spectrum (FTIR). The discoloration efficiency (DE) of free radish peroxidase (FRP) and immobilized (IRP) on the dyes methylene blue (MB) and navy blue (NB) was determined by biodegradation assays conducted at 200 rpm for 1 h at 25°C. The evaluated parameters were: effect of pH, amount of biocatalyst (FRP for MB and NB; IRP for MB and NB), molar ratio dye: H2O2 (hydrogen peroxide) and contact time. Also, operational stability was analyzed. Under optimized conditions [pH 8.0 (MB) and pH 5.0 (NB), amount of biocatalyst to MB: 40.40 U (FRP) and 3.25 × 10−3 U (IRP), and to NB: 13.45 U (FRP) and 26.25 × 10−3 U (IRP), and molar ratio dye: H2O2 to IRP: MB (1:0.5 mmol/L) and NB (1:10 mmol/L)], the maximum DE for IRP was 86% for MB and 61% for NB. IRP can be reused ten times for MB and two times for NB. Thus, the results confirm the potential use of peroxidase of radish immobilized on coconut fiber in the biodegradation of dyes.
| Published in | Chemical and Biomolecular Engineering (Volume 7, Issue 4) |
| DOI | 10.11648/j.cbe.20220704.11 |
| Page(s) | 54-68 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Radish, Coconut Fiber, Immobilization, Biodegradation, Textile Dyes
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APA Style
Kennedy Costa da Conceicao, Patrick Alan Dantas Araujo, Alvaro Silva Lima, Laiza Canielas Krause, Alini Tinoco Fricks, et al. (2023). Biodegradation of Textile Dyes by Radish Peroxidase (Raphanus sativus L.) Immobilized on Coconut Fiber. Chemical and Biomolecular Engineering, 7(4), 54-68. https://doi.org/10.11648/j.cbe.20220704.11
ACS Style
Kennedy Costa da Conceicao; Patrick Alan Dantas Araujo; Alvaro Silva Lima; Laiza Canielas Krause; Alini Tinoco Fricks, et al. Biodegradation of Textile Dyes by Radish Peroxidase (Raphanus sativus L.) Immobilized on Coconut Fiber. Chem. Biomol. Eng. 2023, 7(4), 54-68. doi: 10.11648/j.cbe.20220704.11
AMA Style
Kennedy Costa da Conceicao, Patrick Alan Dantas Araujo, Alvaro Silva Lima, Laiza Canielas Krause, Alini Tinoco Fricks, et al. Biodegradation of Textile Dyes by Radish Peroxidase (Raphanus sativus L.) Immobilized on Coconut Fiber. Chem Biomol Eng. 2023;7(4):54-68. doi: 10.11648/j.cbe.20220704.11
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Download@article{10.11648/j.cbe.20220704.11,
author = {Kennedy Costa da Conceicao and Patrick Alan Dantas Araujo and Alvaro Silva Lima and Laiza Canielas Krause and Alini Tinoco Fricks and Cleide Mara Farias Soares and Rebeca Yndira Cabrera-Padilla},
title = {Biodegradation of Textile Dyes by Radish Peroxidase (Raphanus sativus L.) Immobilized on Coconut Fiber},
journal = {Chemical and Biomolecular Engineering},
volume = {7},
number = {4},
pages = {54-68},
doi = {10.11648/j.cbe.20220704.11},
url = {https://doi.org/10.11648/j.cbe.20220704.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20220704.11},
abstract = {The importance of biodegradation in the context of dye removal is presented as a function of the need to use enzyme immobilization as a key technology for effluent treatment. Immobilization is based on the need to improve the stability and recyclability of the biocatalyst in relation to the free enzyme, in addition to a lower risk of product contamination as an advantage. The present study aims to evaluate the biodegradation of textile dyes using radish peroxidase immobilized by physical adsorption method on coconut fiber, as well as its characterization in relation to pH, temperature, and infrared spectrum (FTIR). The discoloration efficiency (DE) of free radish peroxidase (FRP) and immobilized (IRP) on the dyes methylene blue (MB) and navy blue (NB) was determined by biodegradation assays conducted at 200 rpm for 1 h at 25°C. The evaluated parameters were: effect of pH, amount of biocatalyst (FRP for MB and NB; IRP for MB and NB), molar ratio dye: H2O2 (hydrogen peroxide) and contact time. Also, operational stability was analyzed. Under optimized conditions [pH 8.0 (MB) and pH 5.0 (NB), amount of biocatalyst to MB: 40.40 U (FRP) and 3.25 × 10−3 U (IRP), and to NB: 13.45 U (FRP) and 26.25 × 10−3 U (IRP), and molar ratio dye: H2O2 to IRP: MB (1:0.5 mmol/L) and NB (1:10 mmol/L)], the maximum DE for IRP was 86% for MB and 61% for NB. IRP can be reused ten times for MB and two times for NB. Thus, the results confirm the potential use of peroxidase of radish immobilized on coconut fiber in the biodegradation of dyes.},
year = {2023}
}
TY - JOUR T1 - Biodegradation of Textile Dyes by Radish Peroxidase (Raphanus sativus L.) Immobilized on Coconut Fiber AU - Kennedy Costa da Conceicao AU - Patrick Alan Dantas Araujo AU - Alvaro Silva Lima AU - Laiza Canielas Krause AU - Alini Tinoco Fricks AU - Cleide Mara Farias Soares AU - Rebeca Yndira Cabrera-Padilla Y1 - 2023/01/30 PY - 2023 N1 - https://doi.org/10.11648/j.cbe.20220704.11 DO - 10.11648/j.cbe.20220704.11 T2 - Chemical and Biomolecular Engineering JF - Chemical and Biomolecular Engineering JO - Chemical and Biomolecular Engineering SP - 54 EP - 68 PB - Science Publishing Group SN - 2578-8884 UR - https://doi.org/10.11648/j.cbe.20220704.11 AB - The importance of biodegradation in the context of dye removal is presented as a function of the need to use enzyme immobilization as a key technology for effluent treatment. Immobilization is based on the need to improve the stability and recyclability of the biocatalyst in relation to the free enzyme, in addition to a lower risk of product contamination as an advantage. The present study aims to evaluate the biodegradation of textile dyes using radish peroxidase immobilized by physical adsorption method on coconut fiber, as well as its characterization in relation to pH, temperature, and infrared spectrum (FTIR). The discoloration efficiency (DE) of free radish peroxidase (FRP) and immobilized (IRP) on the dyes methylene blue (MB) and navy blue (NB) was determined by biodegradation assays conducted at 200 rpm for 1 h at 25°C. The evaluated parameters were: effect of pH, amount of biocatalyst (FRP for MB and NB; IRP for MB and NB), molar ratio dye: H2O2 (hydrogen peroxide) and contact time. Also, operational stability was analyzed. Under optimized conditions [pH 8.0 (MB) and pH 5.0 (NB), amount of biocatalyst to MB: 40.40 U (FRP) and 3.25 × 10−3 U (IRP), and to NB: 13.45 U (FRP) and 26.25 × 10−3 U (IRP), and molar ratio dye: H2O2 to IRP: MB (1:0.5 mmol/L) and NB (1:10 mmol/L)], the maximum DE for IRP was 86% for MB and 61% for NB. IRP can be reused ten times for MB and two times for NB. Thus, the results confirm the potential use of peroxidase of radish immobilized on coconut fiber in the biodegradation of dyes. VL - 7 IS - 4 ER -
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