Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria

Chinenye Esther Okoro-Ani; Chima Innocent Ugbor; Stellamaris Ojiuzor Ibhawaegbele; Iniekong Philip Udoh; Chukwuma Paulinus Igweagu; Ogechukwu Calista Dozie-Nwakile; Chima Gabriel Ezeah

doi:doi:10.11648/j.cbe.20210601.12

Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria

Chinenye Esther Okoro-Ani, Chima Innocent Ugbor, Stellamaris Ojiuzor Ibhawaegbele, Iniekong Philip Udoh, Chukwuma Paulinus Igweagu, Ogechukwu Calista Dozie-Nwakile, Chima Gabriel Ezeah

Published in Chemical and Biomolecular Engineering (Volume 6, Issue 1)

Received: 28 August 2020 Accepted: 18 September 2020 Published: 22 March 2021

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Abstract

This study was designed to determine the prevalence of multi-drug resistant tuberculosis (MDR-TB) among pulmonary tuberculosis patients attending NnamdiAzikiwe University Teaching Hospital (NAUTH) Nnewi and St Patrick’s Hospital Mile 4 Abakaliki demographically. Patients with persistent cough for over two weeks were screened by Ziehl-Neelsen ZN technique for the presence of acid fast bacilli (AFB) in their sputum and a total of 103 patients with AFB positive sputum samples were recruited. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid USA) and culture on Lowestein Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay (NRA). Eighty-three 83 (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and out of the 83 (80.6%) samples analysed by Xpert MTB/RIF assay 45 (67.2%) were rifampicin resistant. Age group 26-35 years showed the highest proportion of positive culture results (33.7%) followed by age group 18-25 (28.8%) years. Demographically, age group 26-35 years had a high prevalence rate of MDR-TB (50.0%) and female gender also showed high prevalence rate of MDR-RB (48.5%). Strikingly, educational status was significantly associated with MDR-TB (P=.020). St Patrick’s hospital had a high prevalence rate of MDR-TB (46.94%) when compared with NAUTH (38.9%) and these indicates that there is high prevalence of MDR-TB among patients with pulmonary TB in these sites. The demographic results of this study calls for urgent and serious intervention as MDR-TB prevalence is increasing even in the face of intense national TB control program.

Published in	Chemical and Biomolecular Engineering (Volume 6, Issue 1)
DOI	10.11648/j.cbe.20210601.12
Page(s)	11-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Prevalence, MDR-TB, Demographic Factors, Patient

References

[1]	McGrath M, Geyvan Pi Hius N. C, Van Helden P. D, Warren R. M and Warner D. F (2013) Mutation rate and the emergence of drug resistance in Mycobacterium tuberculosis. Journal of Antimicrobial Chemotherapy; 205: 1-11.
[2]	McBryde E. S, Meehan I. M, Doan T. N, Ragonnet R, Marais J-B Guernier V, Trauer J. M (2017). The risk of global epidemic replacement with drug resistant Mycobacterium tuberculosis strains. International Journal of Infectious Diseases; 2856: 1-7.
[3]	Ricks P. M, Farai M, Surbhi M, Rosalia I, Abbas Z, Lauren A. L, DeLuca N, Krashin J. S, Nakashima A. K and Holtz T. H (2012). Characteristics of multidrug-resistant tuberculosis in Namibia. BMC Infectious Diseases; 12: 385-393.
[4]	Sergeev R., Colijin C., Murray M., Cohen T. (2012). Modelling the dynamic relationship between HIV and drug resistant tuberculosis. Translational Medicine; 4: 135-148.
[5]	Lawson L., Habib A. G., Okobi M. I., Idiong D., Olajide I., Emenyonu N., Onuoha N., Cuevas L. E., Ogiri S. O (2010). Pilot study on multidrug resistant tuberculosis in Nigeria. Annals of African Medicine; 9: 184-187.
[6]	American Lung Association (ALA) (2013). Multiple Drug-Resistant Tuberculosis (MDR-TB) Fact Sheet. Retrieved from: http://www.lung.org/lung-disease/tuberculosis/factsheets/multidrug-resistant.html
[7]	World Health Organization (2013). Global tuberculosis report 2013: http://www.who.int/tb/publications/global_report/en/
[8]	Uzoewulu N. G., Ibeh I. N., Lawson L., Goyal M., Umenyonu N., Ofiaeli R. O., and Okonkwo R. (2014). Drug resistant Mycobacterium tuberculosis in tertiary hospital southeast, Nigeria. Journal of Medical Microbiology and Diagnosis; 3: 141-148.
[9]	(FMH, 2010) Federal Ministry of Health (2010) Department of public health guidelines for community tuberculosis care Stop TB national Tuberculosis and Leprosy control programme Nigeria 6-9.
[10]	Gandhi, N. R., Moll, A., Sturm, A. W., Pawinski, R., Govender, T., Lalloo, U., Zeller, K., Andrews, J. and Friedland, G. (2006). Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet, 368: 1575-1580.
[11]	Manual on Technical SOPs for TB Labs (2012): Nigeria Federal Ministry of Health, Department of Public Health Abuja.
[12]	Federal Republic of Nigeria Official Gazette (2009). National Population Commission Abuja, Nigeria.
[13]	Charan J. and Biswas T. (2013). How to calculate sample size for different study designs in medical research. Indian Journal of Psychological Medicine; 35 (2): 121-126.
[14]	Solomon Weldegebreal Asgedom, Mebrahtu Teweldemedhin, and Hailay Gebreyesus. Prevalence of Multidrug-Resistant Tuberculosis and Associated Factors in Ethiopia: A Systematic Review. Journal of Pathogens, 2018: 8.
[15]	World Health Organization, “WHO report 2013,” Global Tuberculosis control, WHO, Geneva, Switzerland, 2013.
[16]	Olusoji D and Osman E. (2011). Prevalence and risk factors associated with drug resistant TB in South West, Nigeria. Asian Pacific Journal of Tropical Medicine; 17: 148-151.
[17]	Otu A., Umoh V., Habib A., Ameh S., Lawson L., Ansa V. (2013). Drug resistance among pulmonary tuberculosis patients in Calabar, Nigeria. Pulmonary Medicine; 2013: 76-84.
[18]	Gaude G. S, Hattiholli J, Kumar P. (2014) Risk factors and drug-resistance patterns among Pulmonary tuberculosis patients in Northern Karnataka region, India. Nigerian Medical Journal; 55 (4): 327-332.
[19]	World Health Organisation (2012). Nigeria Tuberculosis Fact Sheet. WHO January 2012.
[20]	Das D., Prakasini S, Biswanath M. (2016). First Line Anti-TB Drug Resistance in an Urban Area of Odisha, India. Journal of Clinical and Diagnostic Research. 10 (11): DC04-DC06.
[21]	Vashakldze L., Salakala A, Shubladze N., Cynamon M (2009). Prevalence and risk factors for drug resistance among hospitalized pulmonary tuberculosis patients in Georgia. International Journal of Tuberculosis and Lung diseases; 13 (9): 1148-1153.
[22]	Range. N, Henrik F, Said M, Pascal M, John C, Andrew K, Apolinary M and Aase B. A (2012). Anti-tuberculosis drug resistance pattern among pulmonary tuberculosis patients with or without HIV infection in Mwanza, Tanzania. Tanzania Journal of Health Research; 14 (4): 1-9.
[23]	Lukoye D, Ssengooba W, Musisi K, Kasule G. W, Cobelens F. G. J, Joloba M and Gomez G. B (2015). Variation and risk factors of drug resistant tuberculosis in Sub-Saharan African: a systematic review and meta-analysis. BMC Public Health; 15: 1-13.

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Chinenye Esther Okoro-Ani, Chima Innocent Ugbor, Stellamaris Ojiuzor Ibhawaegbele, Iniekong Philip Udoh, Chukwuma Paulinus Igweagu, et al. (2021). Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chemical and Biomolecular Engineering, 6(1), 11-16. https://doi.org/10.11648/j.cbe.20210601.12

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ACS Style

Chinenye Esther Okoro-Ani; Chima Innocent Ugbor; Stellamaris Ojiuzor Ibhawaegbele; Iniekong Philip Udoh; Chukwuma Paulinus Igweagu, et al. Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chem. Biomol. Eng. 2021, 6(1), 11-16. doi: 10.11648/j.cbe.20210601.12

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AMA Style

Chinenye Esther Okoro-Ani, Chima Innocent Ugbor, Stellamaris Ojiuzor Ibhawaegbele, Iniekong Philip Udoh, Chukwuma Paulinus Igweagu, et al. Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chem Biomol Eng. 2021;6(1):11-16. doi: 10.11648/j.cbe.20210601.12

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@article{10.11648/j.cbe.20210601.12,
  author = {Chinenye Esther Okoro-Ani and Chima Innocent Ugbor and Stellamaris Ojiuzor Ibhawaegbele and Iniekong Philip Udoh and Chukwuma Paulinus Igweagu and Ogechukwu Calista Dozie-Nwakile and Chima Gabriel Ezeah},
  title = {Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria},
  journal = {Chemical and Biomolecular Engineering},
  volume = {6},
  number = {1},
  pages = {11-16},
  doi = {10.11648/j.cbe.20210601.12},
  url = {https://doi.org/10.11648/j.cbe.20210601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210601.12},
  abstract = {This study was designed to determine the prevalence of multi-drug resistant tuberculosis (MDR-TB) among pulmonary tuberculosis patients attending NnamdiAzikiwe University Teaching Hospital (NAUTH) Nnewi and St Patrick’s Hospital Mile 4 Abakaliki demographically. Patients with persistent cough for over two weeks were screened by Ziehl-Neelsen ZN technique for the presence of acid fast bacilli (AFB) in their sputum and a total of 103 patients with AFB positive sputum samples were recruited. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid USA) and culture on Lowestein Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay (NRA). Eighty-three 83 (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and out of the 83 (80.6%) samples analysed by Xpert MTB/RIF assay 45 (67.2%) were rifampicin resistant. Age group 26-35 years showed the highest proportion of positive culture results (33.7%) followed by age group 18-25 (28.8%) years. Demographically, age group 26-35 years had a high prevalence rate of MDR-TB (50.0%) and female gender also showed high prevalence rate of MDR-RB (48.5%). Strikingly, educational status was significantly associated with MDR-TB (P=.020). St Patrick’s hospital had a high prevalence rate of MDR-TB (46.94%) when compared with NAUTH (38.9%) and these indicates that there is high prevalence of MDR-TB among patients with pulmonary TB in these sites. The demographic results of this study calls for urgent and serious intervention as MDR-TB prevalence is increasing even in the face of intense national TB control program.},
 year = {2021}
}

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TY  - JOUR
T1  - Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria
AU  - Chinenye Esther Okoro-Ani
AU  - Chima Innocent Ugbor
AU  - Stellamaris Ojiuzor Ibhawaegbele
AU  - Iniekong Philip Udoh
AU  - Chukwuma Paulinus Igweagu
AU  - Ogechukwu Calista Dozie-Nwakile
AU  - Chima Gabriel Ezeah
Y1  - 2021/03/22
PY  - 2021
N1  - https://doi.org/10.11648/j.cbe.20210601.12
DO  - 10.11648/j.cbe.20210601.12
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20210601.12
AB  - This study was designed to determine the prevalence of multi-drug resistant tuberculosis (MDR-TB) among pulmonary tuberculosis patients attending NnamdiAzikiwe University Teaching Hospital (NAUTH) Nnewi and St Patrick’s Hospital Mile 4 Abakaliki demographically. Patients with persistent cough for over two weeks were screened by Ziehl-Neelsen ZN technique for the presence of acid fast bacilli (AFB) in their sputum and a total of 103 patients with AFB positive sputum samples were recruited. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid USA) and culture on Lowestein Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay (NRA). Eighty-three 83 (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and out of the 83 (80.6%) samples analysed by Xpert MTB/RIF assay 45 (67.2%) were rifampicin resistant. Age group 26-35 years showed the highest proportion of positive culture results (33.7%) followed by age group 18-25 (28.8%) years. Demographically, age group 26-35 years had a high prevalence rate of MDR-TB (50.0%) and female gender also showed high prevalence rate of MDR-RB (48.5%). Strikingly, educational status was significantly associated with MDR-TB (P=.020). St Patrick’s hospital had a high prevalence rate of MDR-TB (46.94%) when compared with NAUTH (38.9%) and these indicates that there is high prevalence of MDR-TB among patients with pulmonary TB in these sites. The demographic results of this study calls for urgent and serious intervention as MDR-TB prevalence is increasing even in the face of intense national TB control program.
VL  - 6
IS  - 1
ER  -

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Author Information

Chinenye Esther Okoro-Ani

Department of Medical Microbiology, Medical Laboratory Services, Enugu State University Teaching Hospital, Enugu, Nigeria
Chima Innocent Ugbor

Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria
Stellamaris Ojiuzor Ibhawaegbele

Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Nigeria
Iniekong Philip Udoh

Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Nigeria
Chukwuma Paulinus Igweagu

Department of Community Medicine, Enugu State University Teaching Hospital, Parklane, Enugu, Nigeria
Ogechukwu Calista Dozie-Nwakile

Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Nigeria
Chima Gabriel Ezeah

Department of Medical Microbiology, Medical Laboratory Services, Enugu State University Teaching Hospital, Enugu, Nigeria

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APA Style

Chinenye Esther Okoro-Ani, Chima Innocent Ugbor, Stellamaris Ojiuzor Ibhawaegbele, Iniekong Philip Udoh, Chukwuma Paulinus Igweagu, et al. (2021). Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chemical and Biomolecular Engineering, 6(1), 11-16. https://doi.org/10.11648/j.cbe.20210601.12

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ACS Style

Chinenye Esther Okoro-Ani; Chima Innocent Ugbor; Stellamaris Ojiuzor Ibhawaegbele; Iniekong Philip Udoh; Chukwuma Paulinus Igweagu, et al. Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chem. Biomol. Eng. 2021, 6(1), 11-16. doi: 10.11648/j.cbe.20210601.12

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AMA Style

Chinenye Esther Okoro-Ani, Chima Innocent Ugbor, Stellamaris Ojiuzor Ibhawaegbele, Iniekong Philip Udoh, Chukwuma Paulinus Igweagu, et al. Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria. Chem Biomol Eng. 2021;6(1):11-16. doi: 10.11648/j.cbe.20210601.12

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@article{10.11648/j.cbe.20210601.12,
  author = {Chinenye Esther Okoro-Ani and Chima Innocent Ugbor and Stellamaris Ojiuzor Ibhawaegbele and Iniekong Philip Udoh and Chukwuma Paulinus Igweagu and Ogechukwu Calista Dozie-Nwakile and Chima Gabriel Ezeah},
  title = {Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria},
  journal = {Chemical and Biomolecular Engineering},
  volume = {6},
  number = {1},
  pages = {11-16},
  doi = {10.11648/j.cbe.20210601.12},
  url = {https://doi.org/10.11648/j.cbe.20210601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210601.12},
  abstract = {This study was designed to determine the prevalence of multi-drug resistant tuberculosis (MDR-TB) among pulmonary tuberculosis patients attending NnamdiAzikiwe University Teaching Hospital (NAUTH) Nnewi and St Patrick’s Hospital Mile 4 Abakaliki demographically. Patients with persistent cough for over two weeks were screened by Ziehl-Neelsen ZN technique for the presence of acid fast bacilli (AFB) in their sputum and a total of 103 patients with AFB positive sputum samples were recruited. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid USA) and culture on Lowestein Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay (NRA). Eighty-three 83 (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and out of the 83 (80.6%) samples analysed by Xpert MTB/RIF assay 45 (67.2%) were rifampicin resistant. Age group 26-35 years showed the highest proportion of positive culture results (33.7%) followed by age group 18-25 (28.8%) years. Demographically, age group 26-35 years had a high prevalence rate of MDR-TB (50.0%) and female gender also showed high prevalence rate of MDR-RB (48.5%). Strikingly, educational status was significantly associated with MDR-TB (P=.020). St Patrick’s hospital had a high prevalence rate of MDR-TB (46.94%) when compared with NAUTH (38.9%) and these indicates that there is high prevalence of MDR-TB among patients with pulmonary TB in these sites. The demographic results of this study calls for urgent and serious intervention as MDR-TB prevalence is increasing even in the face of intense national TB control program.},
 year = {2021}
}

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TY  - JOUR
T1  - Prevalence of MDR-TB Based on Demographic Factors Among Patients Attending Nauth and St Patrick’s Hospital Mile 4 Abakaliki in Southeast Nigeria
AU  - Chinenye Esther Okoro-Ani
AU  - Chima Innocent Ugbor
AU  - Stellamaris Ojiuzor Ibhawaegbele
AU  - Iniekong Philip Udoh
AU  - Chukwuma Paulinus Igweagu
AU  - Ogechukwu Calista Dozie-Nwakile
AU  - Chima Gabriel Ezeah
Y1  - 2021/03/22
PY  - 2021
N1  - https://doi.org/10.11648/j.cbe.20210601.12
DO  - 10.11648/j.cbe.20210601.12
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20210601.12
AB  - This study was designed to determine the prevalence of multi-drug resistant tuberculosis (MDR-TB) among pulmonary tuberculosis patients attending NnamdiAzikiwe University Teaching Hospital (NAUTH) Nnewi and St Patrick’s Hospital Mile 4 Abakaliki demographically. Patients with persistent cough for over two weeks were screened by Ziehl-Neelsen ZN technique for the presence of acid fast bacilli (AFB) in their sputum and a total of 103 patients with AFB positive sputum samples were recruited. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid USA) and culture on Lowestein Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay (NRA). Eighty-three 83 (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and out of the 83 (80.6%) samples analysed by Xpert MTB/RIF assay 45 (67.2%) were rifampicin resistant. Age group 26-35 years showed the highest proportion of positive culture results (33.7%) followed by age group 18-25 (28.8%) years. Demographically, age group 26-35 years had a high prevalence rate of MDR-TB (50.0%) and female gender also showed high prevalence rate of MDR-RB (48.5%). Strikingly, educational status was significantly associated with MDR-TB (P=.020). St Patrick’s hospital had a high prevalence rate of MDR-TB (46.94%) when compared with NAUTH (38.9%) and these indicates that there is high prevalence of MDR-TB among patients with pulmonary TB in these sites. The demographic results of this study calls for urgent and serious intervention as MDR-TB prevalence is increasing even in the face of intense national TB control program.
VL  - 6
IS  - 1
ER  -

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